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通过实时PCR检测空气中的炭疽热孢子体
AIMS: To detect and isolate Bacillus anthracis from the air by a simple
and rapid procedure. METHODS AND RESULTS: One hundred litres of air
were filtered through an air monitor device. After the membrane was
suspended in PBS, spores of B. anthracis were added. The suspension
was plated on Bacillus cereus selective agar (BCA) plates to detect
B. anthracis colonies. The suspension was also heated at 95 degrees
C for 15 min and used for real-time PCR using a Light Cycler system
and anthrax-specific primers. CONCLUSION: A single cell of B. anthracis
was detected by real-time PCR within 1 h and was also isolated on
a BCA plate within two d. SIGNIFICANCE AND IMPACT OF THE STUDY: Our
results provide evidence that anthrax spores from the atmosphere can
be detected rapidly, suggesting that real-time PCR and a Light Cycler
provides a flexible and powerful tool to prevent epidemics.
【引自Lett Appl Microbiol 2001 Sep;33(3):237-40】 |
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