人类基因组绘图序列的高分辨率放射性杂交图谱
[引自Science 2001 Feb 16;291(5507):1298-1302]
We have constructed a physical map of the human genome by using a
panel of 90 whole-genome radiation hybrids (the TNG panel) in conjunction
with 40,322 sequence-tagged sites (STSs) derived from random genomic
sequences as well as expressed sequences. Of 36,678 STSs on the TNG
radiation hybrid map, only 3604 (9.8%) were absent from the unassembled
draft sequence of the human genome. Of 20,030 STSs ordered on the TNG
map as well as the assembled human genome draft sequence and the Celera
assembled human genome sequence, 36% of the STSs had a discrepant order
between the working draft sequence and the Celera sequence. The TNG
map order was identical to one of the two sequence orders in 60% of
these discrepant cases.
